7.5 Punkt izoelektryczny amfolitów | |||||
C0 amfolitu 10/100 | 0,005 | Cz | 0,05 | Ck | 0,05 |
1. Miareczkowanie amfolitu kwasem | |||||
Vkw | pH | Ca [mol/dm3] *1000 | Ck [mol/dm3] *1000 | [H+] [mol/dm3] *1000 | pK1 |
1 | 3,750 | 4,950 | 0,495 | 0,178 | 2,585 |
2 | 3,477 | 4,902 | 0,980 | 0,333 | 2,659 |
3 | 3,346 | 4,854 | 1,456 | 0,451 | 2,763 |
4 | 3,250 | 4,808 | 1,923 | 0,562 | 2,846 |
5 | 3,165 | 4,762 | 2,381 | 0,684 | 2,908 |
6 | 3,095 | 4,717 | 2,830 | 0,804 | 2,972 |
7 | 3,044 | 4,673 | 3,271 | 0,904 | 3,055 |
8 | 2,995 | 4,630 | 3,704 | 1,012 | 3,138 |
9 | 2,945 | 4,587 | 4,128 | 1,135 | 3,219 |
10 | 2,909 | 4,545 | 4,545 | 1,233 | 3,338 |
11 | 2,869 | 4,505 | 4,955 | 1,352 | 3,471 |
12 | 2,842 | 4,464 | 5,357 | 1,439 | 3,698 |
pK1śr | 3,054 | ||||
2. Miareczkowanie amfolitu zasadą | |||||
Vz | pH | Ca [mol/dm3] *1000 | Cz [mol/dm3] *1000 | [OH-] [mol/dm3] *1000 | pK2 |
1 | 8,271 | 4,950 | 0,495 | 0,002 | 9,227 |
2 | 8,503 | 4,902 | 0,980 | 0,003 | 9,107 |
3 | 8,699 | 4,854 | 1,456 | 0,005 | 9,069 |
4 | 8,844 | 4,808 | 1,923 | 0,007 | 9,023 |
5 | 8,973 | 4,762 | 2,381 | 0,009 | 8,976 |
6 | 9,091 | 4,717 | 2,830 | 0,012 | 8,920 |
7 | 9,212 | 4,673 | 3,271 | 0,016 | 8,851 |
8 | 9,335 | 4,630 | 3,704 | 0,022 | 8,746 |
9 | 9,462 | 4,587 | 4,128 | 0,029 | 8,537 |
10 | 9,615 | 4,545 | 4,545 | 0,041 | 7,576 |
11 | 9,781 | 4,505 | 4,955 | 0,060 | - |
12 | 9,925 | 4,464 | 5,357 | 0,084 | - |
pK2śr | 8,803 | ||||
pKizo= | 5,9288 |